Characteristics of the anti-F4/80 antibody clone CI:A3-1 In addition to recognizing kupffer cells, langerhans cells, peritoneal macrophages, and splenic red pulp macrophages, the anti-F4/80 antibody clone CI:A3-1 is distinguished from other F4/80 monoclonal antibodies in that it stains microglia
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LIT.NPA.2013.1 © Copyright Bio-Rad Laboratories, Inc. All rights reserved. Published by AbD Serotec, a Bio-Rad Company, Endeavour House, Langford Lane, Langford Business Park, Kidlington, OX5 1GE. AbD Serotec reagents are for research purposes only, not for therapeutic or diagnostic use. Cy containing products or portions thereof are manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy® and CyDye® are registered trademarks of GE Healthcare Limited. Alexa Fluor® is a registered Fig 1. Liver tissue was fixed then stained with MCA497 after pre-treatment with citrate buffer at ph 6.0. Brown patches represent stained macrophages. Characteristics of the anti-F4/80 antibody clone CI:A3-1 In addition to recognizing kupffer cells, langerhans cells, peritoneal macrophages, and splenic red pulp macrophages, the anti-F4/80 antibody clone CI:A3-1 is distinguished from other F4/80 monoclonal antibodies in that it stains microglia (macrophages of the central nervous system) (Lawson et al. 1990). This may indicate that clone Cl:A3-1 recognizes a different F4/80 epitope from other F4/80 antibody clones.
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